6BJV
CIRV p19 protein in complex with siRNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 70 |
Detector technology | PIXEL |
Collection date | 2015-03-28 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 |
Unit cell lengths | 46.586, 46.997, 54.883 |
Unit cell angles | 109.44, 111.11, 96.78 |
Refinement procedure
Resolution | 46.807 - 2.198 |
R-factor | 0.262 |
Rwork | 0.260 |
R-free | 0.29710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rpu |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.807 | 2.250 |
High resolution limit [Å] | 2.198 | 2.200 |
Rmeas | 0.147 | 0.741 |
Rpim | 0.092 | 0.478 |
Number of reflections | 18756 | 10 |
<I/σ(I)> | 20.7 | 1.84 |
Completeness [%] | 93.8 | 68.9 |
Redundancy | 2.2 | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 3-12% PEG 1500, 100 mM Sodium Acetate pH 4.6, 10-40 mM MgCl2 |