6BJG
CIRV p19 mutant T111H in complex with siRNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 70 |
Detector technology | PIXEL |
Collection date | 2014-12-17 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 |
Unit cell lengths | 46.386, 46.934, 54.623 |
Unit cell angles | 109.33, 111.12, 96.61 |
Refinement procedure
Resolution | 34.826 - 2.290 |
R-factor | 0.2146 |
Rwork | 0.212 |
R-free | 0.25740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rpu |
RMSD bond length | 0.004 |
RMSD bond angle | 0.559 |
Data reduction software | XDS |
Data scaling software | Aimless (0.3.11) |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 34.940 | 34.940 | 2.380 |
High resolution limit [Å] | 2.290 | 8.890 | 2.290 |
Rmerge | 0.054 | 0.028 | 0.145 |
Rmeas | 0.062 | 0.032 | 0.169 |
Rpim | 0.031 | 0.016 | 0.086 |
Number of reflections | 15496 | 272 | 1368 |
<I/σ(I)> | 13.3 | ||
Completeness [%] | 87.4 | 88.9 | 78.2 |
Redundancy | 3.9 | 3.9 | 3.7 |
CC(1/2) | 0.998 | 0.998 | 0.977 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 277.15 | 3-12% PEG 1500, 100 mM Sodium Acetate pH 4.6, 10-40 mM MgCl2 |