6BJC
TPX2_mini decorated GMPCPP-microtubule
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK II |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 0.01 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 3.3 |
| Software | Appion,FREALIGN,REFMAC,SerialEM,CTFFIND,Coot,EMAN |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
