6BIO

Structure of NlpC1 from Trichomonas vaginalis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-01-01
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9537
Spacegroup name	P 21 2 21
Unit cell lengths	53.150, 70.120, 80.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.396 - 1.200
R-factor	0.1651
Rwork	0.164
R-free	0.19170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6bim
RMSD bond length	0.012
RMSD bond angle	1.205
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.400	1.220
High resolution limit [Å]	1.200	1.200
Rpim	0.056	0.538
Number of reflections	94028	4588
<I/σ(I)>	9.7	1.7
Completeness [%]	100.0	100
Redundancy	7.1	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	Morpheus Screen A1