6BIE
MISREADING CHAPERONE-SUBSTRATE COMPLEXES FROM RANDOM NOISE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-03 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 42.960, 42.960, 258.169 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 64.540 - 1.770 |
| R-factor | 0.17899 |
| Rwork | 0.175 |
| R-free | 0.24785 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5INA |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.322 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.540 | 1.800 |
| High resolution limit [Å] | 1.769 | 1.770 |
| Rmerge | 0.062 | 1.340 |
| Number of reflections | 25052 | |
| <I/σ(I)> | 15.3 | 1.6 |
| Completeness [%] | 100.0 | 99.5 |
| Redundancy | 8.7 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 273 | PEG 3000, IMIDAZOLE, ZINC ACETATE, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K |
