6BHW

B. subtilis SsbA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-23
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0782
Spacegroup name	P 41 21 2
Unit cell lengths	97.479, 97.479, 213.595
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.713 - 2.208
R-factor	0.2326
Rwork	0.232
R-free	0.25830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3vdy
RMSD bond length	0.002
RMSD bond angle	0.456
Data reduction software	HKL-2000 (v712)
Data scaling software	HKL-2000 (v712)
Phasing software	PHENIX ((1.12))
Refinement software	PHENIX ((1.12))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.250
High resolution limit [Å]	2.200	2.210
Rmerge	0.128	1.320
Rmeas	0.133	1.366
Number of reflections	52594	2566
<I/σ(I)>	36.4
Completeness [%]	100.0	100
Redundancy	16.1	15.5
CC(1/2)		0.814

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	50% mixture with 50 mM MES pH6.5, 5% PEG 8000, 80 mM magnesium acetate, 200 mM potassium chloride SsbA was incubated with a 1:2 SsbA to dT35 ratio and a-chymotrypsin prior to crystallization