6BHW
B. subtilis SsbA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-23 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0782 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 97.479, 97.479, 213.595 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.713 - 2.208 |
| R-factor | 0.2326 |
| Rwork | 0.232 |
| R-free | 0.25830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vdy |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.456 |
| Data reduction software | HKL-2000 (v712) |
| Data scaling software | HKL-2000 (v712) |
| Phasing software | PHENIX ((1.12)) |
| Refinement software | PHENIX ((1.12)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.250 |
| High resolution limit [Å] | 2.200 | 2.210 |
| Rmerge | 0.128 | 1.320 |
| Rmeas | 0.133 | 1.366 |
| Number of reflections | 52594 | 2566 |
| <I/σ(I)> | 36.4 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 16.1 | 15.5 |
| CC(1/2) | 0.814 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 50% mixture with 50 mM MES pH6.5, 5% PEG 8000, 80 mM magnesium acetate, 200 mM potassium chloride SsbA was incubated with a 1:2 SsbA to dT35 ratio and a-chymotrypsin prior to crystallization |
