6BHS
HIV-1 CA hexamer in complex with IP6, hexagonal crystal form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-10-10 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | P 6 |
Unit cell lengths | 90.649, 90.649, 56.614 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.252 - 1.984 |
R-factor | 0.2381 |
Rwork | 0.236 |
R-free | 0.28420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3h47 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.091 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.030 |
High resolution limit [Å] | 1.980 | 1.980 |
Rmerge | 0.160 | 1.000 |
Rpim | 0.050 | 0.600 |
Number of reflections | 18475 | |
<I/σ(I)> | 15.2 | |
Completeness [%] | 100.0 | |
Redundancy | 11 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8 | 293 | 10% PEG 8,000, 2% Tacsimate, 0.1 M Tris |