6BHS
HIV-1 CA hexamer in complex with IP6, hexagonal crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-10-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 6 |
| Unit cell lengths | 90.649, 90.649, 56.614 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.252 - 1.984 |
| R-factor | 0.2381 |
| Rwork | 0.236 |
| R-free | 0.28420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3h47 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.091 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.160 | 1.000 |
| Rpim | 0.050 | 0.600 |
| Number of reflections | 18475 | |
| <I/σ(I)> | 15.2 | |
| Completeness [%] | 100.0 | |
| Redundancy | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 293 | 10% PEG 8,000, 2% Tacsimate, 0.1 M Tris |
