6BHP

Crystal structure of the Chlamydomonas reinhardtii LCI1 channel

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2015-08-10
Detector	ADSC QUANTUM 315
Wavelength(s)	1.008
Spacegroup name	P 31 2 1
Unit cell lengths	85.235, 85.235, 209.784
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.753 - 3.209
R-factor	0.2229
Rwork	0.219
R-free	0.29680
Structure solution method	SIRAS
RMSD bond length	0.002
RMSD bond angle	0.515
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHELXCD
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.310
High resolution limit [Å]	3.200	6.890	3.200
Rmerge	0.104	0.030
Rmeas	0.110	0.032
Rpim	0.044	0.011	0.546
Total number of observations	143222
Number of reflections	15305
<I/σ(I)>	5.1
Completeness [%]	100.0	99.9	100
Redundancy	9.4	8.5	9.6
CC(1/2)		0.999	0.471

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	298	PEG2000MME, sodium citrate, nickel chloride