6BFM
Galactose-binding Lectin from Mytilus californianus, Isoform1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 101 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-11-16 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5416 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.960, 41.036, 66.224 |
| Unit cell angles | 90.00, 106.55, 90.00 |
Refinement procedure
| Resolution | 36.560 - 1.488 |
| R-factor | 0.1568 |
| Rwork | 0.155 |
| R-free | 0.19080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wmu |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.160 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((dev_2932)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 36.560 |
| High resolution limit [Å] | 1.488 |
| Rmerge | 0.099 |
| Rpim | 0.027 |
| Number of reflections | 48409 |
| <I/σ(I)> | 52.7 |
| Completeness [%] | 99.6 |
| Redundancy | 9.9 |
| CC(1/2) | 0.819 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291.15 | 18% polyethylene glycol 4000, 50 mM sodium acetate, 0.1M HEPES, pH 7.5, 0.1M lithium sulfate |
