6BFH

Structure of the kanamycin complex of aminoglycoside acetyltransferase AAC(6')-Im

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-12-09
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0332
Spacegroup name	P 65
Unit cell lengths	107.750, 107.750, 37.330
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.881 - 1.950
R-factor	0.1717
Rwork	0.171
R-free	0.19430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qc6
RMSD bond length	0.008
RMSD bond angle	0.916
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.700	2.000
High resolution limit [Å]	1.950	1.950
Rmerge	0.066	0.743
Number of reflections	18370	1332
<I/σ(I)>	18.2	3.6
Completeness [%]	99.8	98.4
Redundancy	7.3	7.1
CC(1/2)	0.999	0.894

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.1 M MES pH 6.5, 12% PEG 20000