6BFB
Crystal structure of a F. nucleatum FMN riboswitch bound to WG-3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 71.130, 71.130, 141.190 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.410 - 2.820 |
| R-factor | 0.205 |
| Rwork | 0.203 |
| R-free | 0.24300 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.780 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | autoBUSTER |
| Refinement software | autoBUSTER (2.11.7) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 56.460 |
| High resolution limit [Å] | 2.820 |
| Number of reflections | 9904 |
| <I/σ(I)> | 32 |
| Completeness [%] | 94.3 |
| Redundancy | 9.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 300 | 0.1 M sodium acetate pH 5.5-6.5, 0.2 M MgCl2, 7-11% v/v PEG 4000 |
