6BDX
4-hydroxy tetrahydrodipicolinate reductase from Neisseria gonorrhoeae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-23 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 57.803, 71.723, 138.414 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.850 |
| R-factor | 0.17139 |
| Rwork | 0.169 |
| R-free | 0.20989 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1arz |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.911 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.061 | 0.784 |
| Rmeas | 0.067 | 0.757 |
| Rpim | 0.024 | 0.270 |
| Number of reflections | 25492 | 1251 |
| <I/σ(I)> | 48.2 | 3.38 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.9 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.1 M BIS-TRIS pH 6.5, 2.0 M Ammonium sulfate |
