6BDU
Crystal structure of PprA from Deinococcus radiodurans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-22 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9762 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 56.629, 99.149, 116.690 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.210 - 2.000 |
R-factor | 0.2065 |
Rwork | 0.205 |
R-free | 0.23570 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.688 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.340 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.152 | 1.628 |
Rmeas | 0.159 | 1.606 |
Rpim | 0.061 | 0.662 |
Number of reflections | 45262 | 3281 |
<I/σ(I)> | 11.2 | 1.9 |
Completeness [%] | 100.0 | |
Redundancy | 12.1 | 12.6 |
CC(1/2) | 0.997 | 0.658 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293.15 | Protein at 2.4mg/mL in 150mM KCl, 20mM Tris, pH 7.5 was mixed in 1:1 volume ratio with a solution of 0.2 M Lithium Citrate Tribasic and 20 % (w/v) PEG 3350. The drop was suspended over 1.5M Ammonium sulfate. |