6BDG
HFQ monomer in spacegroup p6 at 1.93 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-003 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-07-16 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 6 |
| Unit cell lengths | 61.370, 61.370, 28.260 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.359 - 1.964 |
| R-factor | 0.1961 |
| Rwork | 0.192 |
| R-free | 0.23270 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.065 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.360 | 2.248 |
| High resolution limit [Å] | 1.960 | 1.964 |
| Number of reflections | 4191 | |
| <I/σ(I)> | 12.7 | |
| Completeness [%] | 94.0 | 100 |
| Redundancy | 2 | 1.9 |
| CC(1/2) | 0.991 | 0.862 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1 M Na/K phosphate pH 6.2 10 % W/V PEG 3000 10 mM Spermidine 5 mM Mg Acetate |
