6BDD

Crystal structure of Fe(II) unliganded H-NOX protein from K. algicida

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2017-05-27
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.977
Spacegroup name	P 21 21 21
Unit cell lengths	48.966, 57.613, 68.796
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.170 - 1.901
R-factor	0.1951
Rwork	0.193
R-free	0.24210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4u99
RMSD bond length	0.002
RMSD bond angle	0.648
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.170	1.930
High resolution limit [Å]	1.900	1.900
Rmerge	0.110	0.670
Number of reflections	15750	692
<I/σ(I)>	9.78	2.41
Completeness [%]	99.0	91.4
Redundancy	6
CC(1/2)	0.616

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.1 M Tris-HCl, pH 8.5, 0.3 M LiCl, 34% (w/v) PEG 6000