6BDC
Structure of Hcp1 from Flavobacterium johnsoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979, 1.075 |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 79.021, 79.021, 82.913 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.456 - 2.496 |
| R-factor | 0.2519 |
| Rwork | 0.248 |
| R-free | 0.28050 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.702 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXDE |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.700 |
| Number of reflections | 4577 |
| <I/σ(I)> | 41.5 |
| Completeness [%] | 98.6 |
| Redundancy | 43.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 21% PEG3350, 200mM sodium citrate, 10% glycerol |
