6BD1
Complex of 14-3-3 theta with an IRSp53 peptide phosphorylated at S366
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-17 |
| Detector | APEX II CCD |
| Wavelength(s) | 1.540 |
| Spacegroup name | P 1 |
| Unit cell lengths | 59.916, 69.301, 84.732 |
| Unit cell angles | 106.11, 95.56, 114.87 |
Refinement procedure
| Resolution | 29.421 - 2.350 |
| R-factor | 0.2135 |
| Rwork | 0.212 |
| R-free | 0.25130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2br9 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.450 |
| Data scaling software | SAINT |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.421 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.058 | 0.205 |
| Number of reflections | 45896 | 4330 |
| <I/σ(I)> | 20 | 4.9 |
| Completeness [%] | 95.6 | 90.4 |
| Redundancy | 5.5 | 2.7 |
| CC(1/2) | 0.998 | 0.889 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291.15 | 0.125 M CaCl2, 12% Peg 3350 |
