6BCG
I-LtrI A28G bound to cognate substrate (pre-cleavage complex)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-10 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.859, 65.942, 169.734 |
| Unit cell angles | 89.99, 90.03, 90.24 |
Refinement procedure
| Resolution | 35.763 - 2.900 |
| R-factor | 0.2649 |
| Rwork | 0.263 |
| R-free | 0.30680 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.537 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.040 | 3.010 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.065 | 0.336 |
| Number of reflections | 80944 | |
| <I/σ(I)> | 6.7 | 2 |
| Completeness [%] | 99.9 | 98.99 |
| Redundancy | 10.6 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M TRIS pH 7.0 20% W/V PEG 2000 MME |
