6BBW
Structure of the major pilin protein (T3.2) from Streptococcus pyogenes serotype GAS13637
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2014-09-23 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 |
| Unit cell lengths | 32.050, 60.570, 72.790 |
| Unit cell angles | 90.77, 91.77, 104.07 |
Refinement procedure
| Resolution | 72.740 - 1.800 |
| R-factor | 0.20022 |
| Rwork | 0.198 |
| R-free | 0.24873 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6bbt |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.376 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | BUSTER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.740 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.039 | 0.296 |
| Number of reflections | 47406 | 2751 |
| <I/σ(I)> | 19.7 | |
| Completeness [%] | 96.4 | 95.4 |
| Redundancy | 3.9 | 3.9 |
| CC(1/2) | 0.999 | 0.937 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 0.2 M NH4H2PO4, 20% w/v PEG 3350 |
