6BBT
Structure of the major pilin protein (T-13) from Streptococcus pyogenes serotype GAS131465
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2014-06-18 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.772, 114.968, 128.711 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 85.740 - 1.900 |
| R-factor | 0.20519 |
| Rwork | 0.204 |
| R-free | 0.23577 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3b2m |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.426 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER (2.5.6) |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.740 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rpim | 0.071 | 0.762 |
| Number of reflections | 61489 | 3877 |
| <I/σ(I)> | 10.4 | 1.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.9 | 14.9 |
| CC(1/2) | 0.997 | 0.461 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 291 | 20% w/v PEG 6000, 0.2 M MOPS pH 7.3 |
