6BBS
Joint X-ray/neutron structure of human carbonic anhydrase II in complex with brinzolamide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Detector | MAATEL |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.755, 44.011, 75.957 |
| Unit cell angles | 90.00, 104.18, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.000 |
| Rwork | 0.276 |
| R-free | 0.28700 |
| Refinement software | nCNS (1.0.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 1.850 |
| Rmerge | 0.062 |
| Number of reflections | 23670 |
| <I/σ(I)> | 14.1 |
| Completeness [%] | 95.7 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 290 | 1.6 M sodium citrate, 50 mM TRIS pH 8.0 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 290 | 1.6 M sodium citrate, 50 mM TRIS pH 8.0 |
