6BBK

N-terminally truncated group I PilA from Pseudomonas aeruginosa strain 1244

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2016-09-19
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	30.494, 42.394, 82.442
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.554 - 1.730
R-factor	0.1882
Rwork	0.186
R-free	0.22590
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.736
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.000	35.000	1.730
High resolution limit [Å]	1.700	4.610	1.700
Rmerge	0.051	0.031	0.218
Total number of observations	434970
Number of reflections	12215	709	453
<I/σ(I)>	19
Completeness [%]	98.8	100	78.2
Redundancy	35.6	34.9	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	0.1M HEPES:NaOH, 20% (w/v) PEG 4000, 10% (v/v) 2-Propanol