6BBG
The CRAC channel Orai in an unlatched-closed conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-06 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.738 |
Spacegroup name | I 41 |
Unit cell lengths | 250.380, 250.380, 210.376 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.983 - 6.900 |
R-factor | 0.3361 |
Rwork | 0.334 |
R-free | 0.35430 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.065 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 7.020 |
High resolution limit [Å] | 6.900 | 6.900 |
Rpim | 0.030 | |
Number of reflections | 11063 | 540 |
<I/σ(I)> | 39.1 | 0.5 |
Completeness [%] | 99.7 | 99.8 |
Redundancy | 11 | 11.6 |
CC(1/2) | 0.123 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 35% PEG 400 100 mM potassium phosphate, pH 7.5 |