6BBG
The CRAC channel Orai in an unlatched-closed conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.738 |
| Spacegroup name | I 41 |
| Unit cell lengths | 250.380, 250.380, 210.376 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.983 - 6.900 |
| R-factor | 0.3361 |
| Rwork | 0.334 |
| R-free | 0.35430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.065 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 7.020 |
| High resolution limit [Å] | 6.900 | 6.900 |
| Rpim | 0.030 | |
| Number of reflections | 11063 | 540 |
| <I/σ(I)> | 39.1 | 0.5 |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 11 | 11.6 |
| CC(1/2) | 0.123 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 35% PEG 400 100 mM potassium phosphate, pH 7.5 |
