6BBF

The CRAC channel Orai in an open conformation; H206A gain-of-function mutation

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-08-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.100000
Spacegroup name	I 41
Unit cell lengths	262.344, 262.344, 220.443
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.982 - 6.706
R-factor	0.3145
Rwork	0.312
R-free	0.33700
RMSD bond length	0.006
RMSD bond angle	1.163
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	6.820
High resolution limit [Å]	6.700	6.700
Rpim	0.011	0.570
Number of reflections	13366	644
<I/σ(I)>	92.4	1.1
Completeness [%]	100.0	100
Redundancy	55.8	58.7
CC(1/2)		0.214

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	38% PEG 400 500 mM NaCl 100 mM Tris-HCl