6BBE

Structure of N-glycosylated porcine surfactant protein-D

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	90
Detector technology	IMAGE PLATE
Collection date	2013-06-20
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 63
Unit cell lengths	66.015, 66.015, 64.658
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	14.699 - 1.898
R-factor	0.2259
Rwork	0.223
R-free	0.25010
RMSD bond length	0.013
RMSD bond angle	0.805
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	15.000	15.000	1.970
High resolution limit [Å]	1.900	4.071	1.900
Rmerge	0.063	0.037	0.376
Total number of observations	79516
Number of reflections	12220	1146	1045
<I/σ(I)>	25.4
Completeness [%]	96.0	87.4	82.2
Redundancy	6.5	6.1	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	Crystallized in hanging drops by mixing 5 ul protein with 5 ul reservoir solution (0.1 M 2-[bis(2-hydroxyethyl)-amino]-2-(hydroxymethyl)propane-1,3-diol, pH 6.5, 0.2 M MgCl2, 15% glycerol, and 22%-20% PEG 3350)