6BBD

Structure of N-glycosylated porcine surfactant protein-D complexed with glycerol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	90
Detector technology	IMAGE PLATE
Collection date	2012-09-05
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 63
Unit cell lengths	66.798, 66.798, 64.974
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	14.852 - 1.898
R-factor	0.2053
Rwork	0.201
R-free	0.24680
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	4dn8
RMSD bond length	0.014
RMSD bond angle	1.085
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	15.000	15.000	1.970
High resolution limit [Å]	1.900	4.070	1.900
Rmerge	0.058	0.034	0.356
Total number of observations	58032
Number of reflections	12304	1232	871
<I/σ(I)>	23.2
Completeness [%]	94.0	91.7	67.4
Redundancy	4.7	5.4	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	Crystallized in hanging drops by mixing 5 ul protein with 5 ul reservoir solution (0.1 M 2-[bis(2-hydroxyethyl)-amino]-2-(hydroxymethyl)propane-1,3-diol, pH 6.5, 0.2 M MgCl2, 15% glycerol, and 22%-20% PEG 3350)