6BB8
Crystal Structure of Frequency-Interacting RNA helicase (FRH)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL14-1 |
Synchrotron site | SSRL |
Beamline | BL14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-06-01 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 1.19499 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 117.770, 117.770, 180.414 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.069 - 3.490 |
R-factor | 0.2532 |
Rwork | 0.251 |
R-free | 0.30060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4u4c |
RMSD bond length | 0.006 |
RMSD bond angle | 1.446 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.650 |
High resolution limit [Å] | 3.490 | 7.580 | 3.520 |
Rmerge | 0.139 | 0.033 | 4.018 |
Rmeas | 0.142 | 0.033 | 4.111 |
Rpim | 0.030 | 0.007 | 0.864 |
Number of reflections | 18697 | 1982 | 1800 |
<I/σ(I)> | 4.6 | 0.93 | |
Completeness [%] | 100.0 | 99.8 | 100 |
Redundancy | 22.1 | 20.6 | 22.4 |
CC(1/2) | 1.000 | 0.564 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.59 | 298 | Sodium Citrate, PEG 3350 |