6BB8

Crystal Structure of Frequency-Interacting RNA helicase (FRH)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-06-01
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.19499
Spacegroup name	P 31 2 1
Unit cell lengths	117.770, 117.770, 180.414
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.069 - 3.490
R-factor	0.2532
Rwork	0.251
R-free	0.30060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4u4c
RMSD bond length	0.006
RMSD bond angle	1.446
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.650
High resolution limit [Å]	3.490	7.580	3.520
Rmerge	0.139	0.033	4.018
Rmeas	0.142	0.033	4.111
Rpim	0.030	0.007	0.864
Number of reflections	18697	1982	1800
<I/σ(I)>	4.6		0.93
Completeness [%]	100.0	99.8	100
Redundancy	22.1	20.6	22.4
CC(1/2)		1.000	0.564

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.59	298	Sodium Citrate, PEG 3350