6BAP
Stigmatella aurantiaca bacterial phytochrome PAS-GAF-PHY, T289H mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.987 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 83.310, 83.310, 475.050 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.284 - 2.650 |
R-factor | 0.2448 |
Rwork | 0.241 |
R-free | 0.31480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6bao |
RMSD bond length | 0.013 |
RMSD bond angle | 1.572 |
Data reduction software | HKL-2000 |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.600 | 2.720 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmeas | 0.084 | 1.081 |
Rpim | 0.041 | 0.553 |
Number of reflections | 56756 | 4109 |
<I/σ(I)> | 7.9 | 0.8 |
Completeness [%] | 99.1 | 99.1 |
Redundancy | 3.5 | 3.3 |
CC(1/2) | 0.997 | 0.364 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 289 | 11 mg/mL protein in 0.1 M MES, pH 6.2, 8-5% w/v PEG20000, 5.2% v/v acetonitrile |