6BAO
Stigmatella aurantiaca phytochrome photosensory core module, wild type
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.987 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 82.510, 134.990, 113.180 |
| Unit cell angles | 90.00, 105.90, 90.00 |
Refinement procedure
| Resolution | 38.400 - 2.180 |
| R-factor | 0.2217 |
| Rwork | 0.220 |
| R-free | 0.26220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4q0j |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.350 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.000 | 2.240 |
| High resolution limit [Å] | 2.180 | 2.180 |
| Rmerge | 1.500 | |
| Rpim | 0.687 | |
| Number of reflections | 69342 | 3403 |
| <I/σ(I)> | 6.7 | 0.6 |
| Completeness [%] | 98.0 | 75 |
| Redundancy | 4.8 | 3.1 |
| CC(1/2) | 0.387 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 289 | 11 mg/mL protein in 0.1 M MES, pH 6.2, 8-5% w/v PEG20000, 5.2% v/v acetonitrile |
