6BAF

Structure of the chromophore binding domain of Stigmatella aurantiaca phytochrome P1, wild-type

Replaces:  4RPW

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.987
Spacegroup name	P 32 2 1
Unit cell lengths	131.689, 131.689, 96.071
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.502 - 1.850
R-factor	0.2117
Rwork	0.211
R-free	0.22830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4RPW
RMSD bond length	0.016
RMSD bond angle	1.743
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	13.900	2.300
High resolution limit [Å]	2.230	2.230
Rmerge	0.084	1.070
Rpim	0.073	0.900
Number of reflections	81078	4168
<I/σ(I)>	6.3	0.6
Completeness [%]	99.0	99
Redundancy	4.4	3.7
CC(1/2)	0.995	0.360

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	280	65 mM Tris-HCl, pH 8.5, 5.2% w/v PEG8000, 35% v/v glycerol