6BA4
Crystal structure of MYST acetyltransferase domain in complex with Acetyl-CoA cofactor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-08-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.363, 57.925, 120.045 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.681 - 1.949 |
| R-factor | 0.1855 |
| Rwork | 0.184 |
| R-free | 0.21280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.223 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.360 | 2.000 |
| High resolution limit [Å] | 1.949 | 1.949 |
| Rmerge | 0.063 | 0.618 |
| Rmeas | 0.068 | 0.666 |
| Rpim | 0.025 | 0.247 |
| Number of reflections | 24350 | 1662 |
| <I/σ(I)> | 19 | 3 |
| Completeness [%] | 99.9 | 99.4 |
| Redundancy | 7.1 | 7.1 |
| CC(1/2) | 0.999 | 0.902 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 292 | 2 M NaCl, 0.1 M Tris pH 7.0, 0.22 M MgCl2 |
