6BA0
Pyrimidine-specific Ribonucleoside Hydrolase from Gardnerella vaginalis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-07-14 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.9537 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 83.070, 79.360, 115.990 |
Unit cell angles | 90.00, 99.62, 90.00 |
Refinement procedure
Resolution | 61.870 - 2.030 |
R-factor | 0.1879 |
Rwork | 0.186 |
R-free | 0.21840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1mas |
RMSD bond length | 0.010 |
RMSD bond angle | 1.387 |
Data reduction software | MOSFLM (7.2.1) |
Data scaling software | Aimless (0.5.32) |
Phasing software | PHASER (2.7.17) |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 61.870 | 61.870 | 2.060 |
High resolution limit [Å] | 2.030 | 11.120 | 2.030 |
Rmerge | 0.198 | 0.076 | 0.942 |
Rmeas | 0.213 | 0.082 | 1.021 |
Rpim | 0.079 | 0.032 | 0.387 |
Number of reflections | 96089 | 626 | 4701 |
<I/σ(I)> | 8.5 | ||
Completeness [%] | 99.9 | 98.7 | 99.2 |
Redundancy | 7.1 | 6.1 | 6.6 |
CC(1/2) | 0.992 | 0.995 | 0.648 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4 | 290 | 0.1M Sodium Acetate 0.2M Ammonium Sulfate 20% (w/v) PEG4000 |