6B9H

Complex of Hook Domain with a Dynein Light Intermediate Chain Peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97750
Spacegroup name	P 21 21 21
Unit cell lengths	31.715, 35.372, 126.149
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.540 - 1.500
R-factor	0.1561
Rwork	0.155
R-free	0.17910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5j8e
RMSD bond length	0.011
RMSD bond angle	1.377
Data reduction software	HKL-2000 (v701.c3)
Data scaling software	HKL-2000 (v701.c3)
Phasing software	PHENIX (1.12_2829)
Refinement software	PHENIX ((1.12_2829))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.550
High resolution limit [Å]	1.500	1.500
Rmerge	0.053	0.373
Number of reflections	23303	2089
<I/σ(I)>	50.7	3.8
Completeness [%]	98.7	90.9
Redundancy	11.2	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.25	293	1.44 M Ammonium Citrate Tribasic