6B9G

human ATL1 GTPase domain bound to GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-09-24
Detector	ADSC QUANTUM 210
Wavelength(s)	0.976
Spacegroup name	F 2 3
Unit cell lengths	308.032, 308.032, 308.032
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.700 - 3.000
R-factor	0.171
Rwork	0.170
R-free	0.20520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3q5d
RMSD bond length	0.002
RMSD bond angle	0.534
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX ((1.12_2829))

Data quality characteristics

	Overall
Low resolution limit [Å]	48.700
High resolution limit [Å]	3.000
Rmerge	0.197
Rmeas	0.201
Number of reflections	48244
<I/σ(I)>	22.8
Completeness [%]	99.9
Redundancy	25.5
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.2 M DL-malic acid pH 7, 20% PEG3350, 1 mM GDP, 2 mM MgCl2