6B9C
Superfolder Green Fluorescent Protein with 4-nitro-L-phenylalanine at the chromophore (position 66)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-04-02 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 55.438, 55.438, 165.746 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.079 - 1.695 |
R-factor | 0.1626 |
Rwork | 0.161 |
R-free | 0.19460 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2b3p |
RMSD bond length | 0.007 |
RMSD bond angle | 1.082 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12rc2_2821: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.730 |
High resolution limit [Å] | 1.695 | 1.695 |
Number of reflections | 28838 | 1396 |
<I/σ(I)> | 28 | 3.4 |
Completeness [%] | 97.8 | 96.5 |
Redundancy | 7.9 | 8.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 1.4 M DL-Malic acid |