6B8Q

Crystal Structure of the Mg2+/CaM:Kv7.5 (KCNQ5) AB domain complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	80
Detector technology	PIXEL
Collection date	2016-06-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	70.821, 116.845, 119.812
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.738 - 2.600
R-factor	0.2245
Rwork	0.222
R-free	0.26960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6b8l
RMSD bond length	0.004
RMSD bond angle	0.533
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.600
Number of reflections	31294
<I/σ(I)>	8.6
Completeness [%]	99.9
Redundancy	9.3
CC(1/2)	0.996	0.253

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.2M magnesium formate, 20% PEG 3350