6B8M
Crystal Structure of the Ca2+/CaM:Kv7.4 (KCNQ4) AB Domain Complex, 1 mM CaCl2 soak
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2016-08-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 108.243, 143.853, 164.272 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.942 - 2.300 |
R-factor | 0.2294 |
Rwork | 0.228 |
R-free | 0.26210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6b8l |
RMSD bond length | 0.003 |
RMSD bond angle | 0.504 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 14.942 | 14.942 | 2.440 |
High resolution limit [Å] | 2.300 | 6.330 | 2.300 |
Rmerge | 0.195 | 0.034 | 2.234 |
Rmeas | 0.215 | 0.037 | 2.482 |
Number of reflections | 109615 | 4904 | 17352 |
<I/σ(I)> | 7.88 | 39.16 | 0.74 |
Completeness [%] | 99.6 | 92.4 | 99.9 |
Redundancy | 5.512 | 5.596 | 5.274 |
CC(1/2) | 0.996 | 0.999 | 0.498 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 2M ammonium sulfate, 0.1M BisTris pH 6.5, 1mM CaCl2 |