6B8K
Crystal structure of Human galectin-3 CRD in complex with Lactulose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-10-13 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.953 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 36.170, 57.870, 62.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.670 - 1.280 |
R-factor | 0.165 |
Rwork | 0.163 |
R-free | 0.19525 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.029 |
RMSD bond angle | 2.679 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.670 |
High resolution limit [Å] | 1.280 |
Number of reflections | 34451 |
<I/σ(I)> | 23.4 |
Completeness [%] | 99.6 |
Redundancy | 12.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 31% PEG6000, 100 mM magnesium chloride, 8 mM BME, 100 mM Tris-HCl, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |