6B8D

1.78 Angstrom Resolution Crystal Structure of N-terminal Fragment (residues 1-405) of Elongation Factor G from Haemophilus influenzae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2017-08-08
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97856
Spacegroup name	C 2 2 21
Unit cell lengths	84.823, 88.487, 130.279
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.800 - 1.780
R-factor	0.17457
Rwork	0.173
R-free	0.21366
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tv2
RMSD bond length	0.008
RMSD bond angle	1.368
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MoRDa
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.810
High resolution limit [Å]	1.780	1.780
Rmerge	0.043	0.797
Rpim	0.018	0.325
Number of reflections	47575	2388
<I/σ(I)>	38.4	2.6
Completeness [%]	99.9	100
Redundancy	6.9	6.9
CC(1/2)		0.832

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	295	11.0 mg/mL protein in 0.5 M sodium chloride, 0.01 M Tris-HCl, pH 8.3 against screen: Classics II (F6), 0.2 M ammonium sulfate, 0.1 M Bis-Tris, pH 5.5, 25% w/v PEG3350