6B8C

Crystal structure of NlpC/p60 domain of peptidoglycan hydrolase SagA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	298
Detector technology	PIXEL
Collection date	2017-03-16
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97930
Spacegroup name	P 4 3 2
Unit cell lengths	100.385, 100.385, 100.385
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.894 - 2.403
R-factor	0.2426
Rwork	0.239
R-free	0.26920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fdy
RMSD bond length	0.003
RMSD bond angle	0.640
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.400
Rpim	0.035
Number of reflections	8107
<I/σ(I)>	38.5
Completeness [%]	100.0
Redundancy	10.2
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	298	2 M ammonium sulfate, 0.1 M Bis-Tris