6B7U
Structure of hen egg-white lysozyme without high-pressure pre-treatment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE W01B-MX2 |
Synchrotron site | LNLS |
Beamline | W01B-MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-05-25 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.459 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 78.079, 78.079, 36.945 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.395 - 1.581 |
R-factor | 0.172 |
Rwork | 0.170 |
R-free | 0.21050 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.366 |
Data scaling software | XSCALE |
Phasing software | SHELX |
Refinement software | PHENIX (1.8.3) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 39.040 |
High resolution limit [Å] | 1.580 |
Rmerge | 0.045 |
Number of reflections | 15337 |
<I/σ(I)> | 38.9 |
Completeness [%] | 94.9 |
Redundancy | 14.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.7M sodium chloride, 0.1M sodium acetate pH 4.8 |