6B7Q
Crystal structure of Legionella effector protein sdeA (lpg2157) aa. 211-910
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-05-06 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9789 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 69.806, 80.616, 85.612 |
Unit cell angles | 90.00, 109.87, 90.00 |
Refinement procedure
Resolution | 80.510 - 2.200 |
R-factor | 0.19731 |
Rwork | 0.195 |
R-free | 0.24078 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.813 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXD |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 80.510 |
High resolution limit [Å] | 2.200 |
Number of reflections | 18728 |
<I/σ(I)> | 7.98 |
Completeness [%] | 99.3 |
Redundancy | 3.278 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 298 | PEG 8,000 9%, 0.1M Hepes pH7.6 |