6B7M
Crystal structure of Legionella effector sdeD (lpg2509) in complex with Ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-17 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9789 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.765, 58.593, 74.124 |
| Unit cell angles | 90.00, 114.66, 90.00 |
Refinement procedure
| Resolution | 67.360 - 1.700 |
| R-factor | 0.1618 |
| Rwork | 0.160 |
| R-free | 0.19565 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.033 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 67.360 |
| High resolution limit [Å] | 1.700 |
| Number of reflections | 108100 |
| <I/σ(I)> | 25.4 |
| Completeness [%] | 99.4 |
| Redundancy | 3.36 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 200 mM NaCl, 100 mM Imidazole pH 7.0, 24% PEG 8000 |
