6B7K

GH43 Endo-Arabinanase from Bacillus licheniformis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-11-20
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	64.558, 124.178, 69.231
Unit cell angles	90.00, 91.16, 90.00

Refinement procedure

Resolution	44.518 - 2.550
R-factor	0.2317
Rwork	0.230
R-free	0.27350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1uv4
RMSD bond length	0.003
RMSD bond angle	0.577
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.12rc1_2807)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.740	2.660
High resolution limit [Å]	2.550	2.550
Rmerge	0.077	0.324
Rmeas	0.108	0.457
Rpim	0.076	0.322
Number of reflections	27463	3271
<I/σ(I)>	7.9	1.5
Completeness [%]	77.3	75.6
Redundancy	2.1	2
CC(1/2)	0.979	0.756

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.05	291	0.1 mM HEPES pH 7.05, 10% PEG 4000 (w/v), 5% 2-propanol (v/v)