6B6R
Orthorhombic trypsin cryocooled to 100 K with 50% mpd as cryoprotectant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | OXFORD DIFFRACTION NOVA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-04-12 |
Detector | OXFORD ONYX CCD |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.115, 58.195, 66.495 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 12.915 - 2.000 |
R-factor | 0.122356255693 |
Rwork | 0.120 |
R-free | 0.17191 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.859 |
Data reduction software | CrysalisPro (1.10.1_2155) |
Data scaling software | SCALA |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 13.900 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 14445 | 2046 |
<I/σ(I)> | 29 | 3.9 |
Completeness [%] | 98.5 | |
Redundancy | 4.1 | |
CC(1/2) | 1.000 | 1.000 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | Well: 25% PEG 8K, 0.2 M AmSO4, 0.1 M benzamidine Hal, 0.1 M Tris buffer pH 8.0 Protein: 40 mg/mL in water |