6B6P
Orthorhombic trypsin cryocooled to 100 K with 30% xylose as cryoprotectant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | OXFORD DIFFRACTION NOVA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-04-13 |
Detector | OXFORD ONYX CCD |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.646, 58.315, 66.411 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 13.381 - 2.000 |
R-factor | 0.124277998421 |
Rwork | 0.122 |
R-free | 0.17094 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.950 |
Data reduction software | CrysalisPro |
Data scaling software | SCALA |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 13.800 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 14610 | 2092 |
<I/σ(I)> | 28.4 | 16.1 |
Completeness [%] | 98.4 | |
Redundancy | 3.3 | |
CC(1/2) | 1.000 | 1.000 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | Well: 25% PEG 8K, 0.2 M AmSO4, 0.1 M benzamidine Hal, 0.1 M Tris buffer pH 8.0 Protein: 40 mg/mL in water |