6B6M
Cyanase from Serratia proteamaculans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.95374 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 136.560, 94.130, 89.110 |
| Unit cell angles | 90.00, 125.49, 90.00 |
Refinement procedure
| Resolution | 43.533 - 1.910 |
| R-factor | 0.1621 |
| Rwork | 0.160 |
| R-free | 0.20190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.814 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.533 | 2.000 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmeas | 0.123 | 0.833 |
| Number of reflections | 69947 | 8089 |
| <I/σ(I)> | 10.26 | |
| Completeness [%] | 97.9 | 88.3 |
| Redundancy | 4 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6.5 | 298 | 0.1M magnesium acetate 10%PEG10000 0.1M MES |
