6B6J
Structure of profilin Art v4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-04-07 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 31 |
| Unit cell lengths | 32.675, 32.675, 81.782 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.300 - 1.900 |
| R-factor | 0.16976 |
| Rwork | 0.167 |
| R-free | 0.21411 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5em0 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.703 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.055 | 0.327 |
| Rmeas | 0.065 | 0.368 |
| Rpim | 0.040 | 0.233 |
| Number of reflections | 7348 | 374 |
| <I/σ(I)> | 27.99 | 3.52 |
| Completeness [%] | 95.5 | 97.7 |
| Redundancy | 2.3 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.9 | 298 | 0.49 M Sodium phosphate monobasic monohydrate |
