6B61

IMPase (AF2372) with 25 mM Asp

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	Cu FINE FOCUS
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2010-11-18
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.0
Spacegroup name	P 32
Unit cell lengths	89.619, 89.619, 102.375
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.731 - 2.700
R-factor	0.1809
Rwork	0.179
R-free	0.22500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lvb
RMSD bond length	0.008
RMSD bond angle	1.076
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall
Low resolution limit [Å]	44.750
High resolution limit [Å]	2.000
Number of reflections	62169
<I/σ(I)>	2.161
Completeness [%]	100.0
Redundancy	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	100 mM myo inositol 400 mM Aspartate 12 % PEG 3350 200 mM Potassium Sulfate 100 mM Magnesium Chloride 100 mM HEPES pH7.0