6B61
IMPase (AF2372) with 25 mM Asp
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | Cu FINE FOCUS |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2010-11-18 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.0 |
Spacegroup name | P 32 |
Unit cell lengths | 89.619, 89.619, 102.375 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.731 - 2.700 |
R-factor | 0.1809 |
Rwork | 0.179 |
R-free | 0.22500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1lvb |
RMSD bond length | 0.008 |
RMSD bond angle | 1.076 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 44.750 |
High resolution limit [Å] | 2.000 |
Number of reflections | 62169 |
<I/σ(I)> | 2.161 |
Completeness [%] | 100.0 |
Redundancy | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 100 mM myo inositol 400 mM Aspartate 12 % PEG 3350 200 mM Potassium Sulfate 100 mM Magnesium Chloride 100 mM HEPES pH7.0 |