6B60
IMPase (AF2372) with 25 mM Glutamate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | Cu FINE FOCUS |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-10-07 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 |
| Unit cell lengths | 89.201, 89.201, 102.041 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.130 - 2.700 |
| R-factor | 0.1947 |
| Rwork | 0.191 |
| R-free | 0.23770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lbv |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.575 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 31.130 |
| High resolution limit [Å] | 2.700 |
| Number of reflections | 24763 |
| <I/σ(I)> | 2.302 |
| Completeness [%] | 99.4 |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 100 mM myo-inositol 25 mM Glutamate 25% PEG 3350 200 mM Potassium sulfate 4 mM Magnesium Chloride |
